Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry

作者: Dominique Dambier , Hamid Benyahia , Giovanni Pensabene-Bellavia , Yildiz Aka Kaçar , Yann Froelicher

DOI: 10.1007/S00299-010-1000-Z

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摘要: The prevalence of sour orange rootstock in the southern and eastern part Mediterranean Basin is presently threatened by spread Citrus Tristeza Virus (CTV) its main vector Toxoptera citricida, combined with abiotic constraints such as drought, salinity alkalinity. search for alternative CTV-resistant rootstocks that also withstand other now considered an urgent priority a sustainable citrus industry area. Complementary progenitors can be found germplasm to combine desired traits, particularly between Poncirus genera. production somatic hybrids allows cumulating all dominant traits irrespective their heterozygosity level, would appear effective way solve challenge facing industry. This paper presents results obtained during regional collaborative effort five countries, develop new hybridization. New embryogenic callus lines used hybridization have been created. Protoplast fusions performed at CIRAD IVIA laboratories, focusing on intergeneric combinations. Analysis ploidy level flow cytometry molecular markers confirmed acquisition interesting tetraploid six Diploid cybrids (Citrus × Poncirus) nucleus C. reticulata or C. aurantifolia mitochondria were identified four agronomical performance pre-existing hybrid trifoliata was validated calcareous soils Morocco. Somatic integrated into breeding programs countries.

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