Effects of Acute γ-Hexachlorocyclohexane Intoxication in Relation to the Redox Regulation of Nuclear Factor-κB, Cytokine Gene Expression, and Liver Injury in the Rat

摘要: γ-Hexachlorocyclohexane-induced hepatotoxicity is associated with oxidative stress. We tested the hypothesis that γ-hexachlorocyclohexane triggers redox activation of nuclear factor-κB (NF-κB), leading to proinflammatory cytokine expression. Liver NF-κB (electrophoretic mobility shift assay), tumor necrosis factor-α (TNF-α) and interleukin-1α (IL-1α) mRNA expression (reverse transcription-polymerase chain reaction), their serum levels (enzyme-linked immunosorbent assay) were measured at different times after treatment (50 mg/kg). The relationship between these hepatic O2 uptake, glutathione protein carbonyl levels, sinusoidal lactate dehydrogenase (LDH) efflux in liver perfusion studies was determined. γ-Hexachlorocyclohexane increased DNA binding 14-22 h treatment, concomitantly significant depletion an increase rate consumption, content carbonyls, LDH efflux. In...