Transient coexpression of nestin, GFAP, and vascular endothelial growth factor in mature reactive astroglia following neural grafting or brain wounds.

摘要: Abstract The spatial and temporal immunoexpression of the intermediate filament (IF) protein nestin its relationship to glial fibrillary acidic (GFAP), vascular endothelial growth factor (VEGF), receptor flt-1 (VEGF-R1) in reactive astroglia was examined following stab wounds or transplants fetal CNS tissue into adult brain. Since developmentally regulated proteins gene transcripts can be reexpressed certain brain injuries, we analyzed profile these experimental paradigms order more fully understand nature gliotic “scar.” Nestin expression transiently up-regulated some but not all astrocytes which often had a different morphology than typical stout, stellate GFAP (+) cells; processes cells tended slender elongated. In from mature brain, robust generally localized wound graft site, peaked at 7–10 days postoperative, absent by 28 days, whereas were far widespread persisted for many months. Only strongly expressed immediately adjacent early wounds, located further sites. contrast, there marked nestin/GFAP colocalization graft/host interface. Semiquantitative analysis combined with confocal microscopy revealed unique compartmentalization expression; single could entirely (+), show coexpression. At 4, 7, 14 41, 58, 32% immunoexpression, respectively, accounted interface, it essentially undetectable postoperative. situ hybridization studies showed within primarily between 4 10 postoperative days. Many astrocytes, as shown electron microscopy, closely related vasculature. Therefore an cell mitogen associated angiogenesis. colocalized VEGF (7%) prominently (25%). Early astroglial activation may involve several IF components possibly distinct astrocytic population that shows rapid, transient injury Expression phenotype affected surgical vicinity indicative reversion immature might less susceptible attendant hypoxia after injury. injured are well known express bioactive compounds, such reexpression early, hallmark elaboration factors VEGF.