Triacylglycerol lipolysis is linked to sphingolipid and phospholipid metabolism of the yeast Saccharomyces cerevisiae.

摘要: Previous work from our laboratory had demonstrated that deletion of TGL3 encoding the major yeast triacylglycerol (TAG) lipase resulted in decreased mobilization TAG, a sporulation defect and changed pattern fatty acids, especially increased amounts C22:0 C26:0 very long chain acids TAG fraction K. Athenstaedt G. Daum, J. Biol. Chem. 278 (2003) 23317-23323]. To study possible link between lipolysis membrane lipid biosynthesis, we carried out metabolic labeling experiments with wild type strains bearing defects three lipases, Tgl3p, Tgl4p Tgl5p. Using H-3]inositol. P-32]orthophosphate, 3H]palmitate C-14]acetate as precursors for complex lipids tgl mutants lower level sphingolipids glycerophospholipids than type. ESI-MS/MS analyses confirmed accumulation these mutant cells reduced phospholipids sphingolipids. In vitro vivo revealed markedly affected flux required sphingolipid glycerophospholipid synthesis. Activity expression acid elongases, Elo1p Elo2p were enhanced consequence lipolysis. Finally, phosphatidylcholine, phosphatidylethanolamine phosphatidylserine molecular species was altered strain underlining important role turnover maintaining pool size compounds remodeling lipids. (C) 2010 Elsevier B.V. All rights reserved.