Normal Thyroid Thermogenesis but Reduced Viability and Adiposity in Mice Lacking the Mitochondrial Glycerol Phosphate Dehydrogenase

摘要: The mitochondrial glycerol phosphate dehydrogenase (mGPD) is important for metabolism of gluconeogenesis or energy production and has been implicated in thermogenesis induced by cold thyroid hormone treatment. mGPD combination with the cytosolic (cGPD) proposed to form shuttle, catalyzing interconversion dihydroxyacetone net oxidation NADH. We made a targeted deletion Gdm1 produced mice lacking mGPD. On C57BL/6J background these showed 50% reduction viability compared wild-type littermates. Uncoupling protein-1 mRNA levels brown adipose tissue did not differ between knockout control pups, suggesting normal thermogenesis. Pups had decreased liver ATP slightly increased phosphate. In contrast, muscle metabolites were adult animals. Adult animals tolerance, circadian rhythm body temperature, demonstrated temperature increase response hormone. However, they found have lower mass index, 40% weight white tissue, fasting blood glucose than controls. phenotype may be secondary consequences obligatory NADH from animal. conclude that, although essential thermogenesis, variations its function affect adiposity mice.