Rapid virus detection procedure for molecular tracing of shellfish associated with disease outbreaks.

摘要: Detection of pathogenic viruses in oysters implicated gastroenteritis outbreaks is often hampered by time-consuming, specialist virus extraction methods. Five RNA methods were evaluated with respect to performance characteristics and sensitivity on artificially contaminated oyster digestive glands. The two most promising procedures further bioaccumulated naturally oysters. efficient method was used trace the source an outbreak situation. Out five protocols, PEG precipitation RNeasy Kit performed best norovirus genogroup III-spiked Analyzing 24-h revealed a slightly better precipitation, but less prone concentrate inhibitors. latter procedure demonstrated presence human noroviruses outbreak. In this outbreak, four out nine individually analyzed glands, detected. one fecal samples clinical cases, identical strains A standard rapid using appeared be useful tracing shellfish as outbreaks, able make effective timely risk management decisions.