Region-specific saturation germline mutagenesis in mice using the Sleeping Beauty transposon system
作者： Vincent W Keng , Kojiro Yae , Tomoko Hayakawa , Sumi Mizuno , Yoshihiro Uno
DOI: 10.1038/NMETH795
关键词:
摘要: Recent consolidation of the whole-genome sequence with genome-wide transcriptome profiling revealed existence functional units within genome in specific chromosomal regions, as seen coordinated expression gene clusters and colocalization functionally related genes. An efficient region-specific mutagenesis screen would greatly facilitate research addressing importance these clusters. Here we use 'local hopping' phenomenon a DNA-type transposon, Sleeping Beauty (SB), for saturation mutagenesis. A transgenic mouse containing both transposon (acts mutagen) transposase (recognizes mobilizes transposon) was bred germ-cell transposition events, allowing us to generate many mutant mice. All genes 4-Mb region original donor site were mutated by SB, indicating potential this system genomic studies region.
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