Protein–Protein Interaction in the -Omics Era: Understanding Mycobacterium tuberculosis Function

摘要: An important challenge for TB investigators in the postgenomic era is to integrate distinct functional strategies study molecular mechanism of Mycobacterium tuberculosis (Mtb) virulence. However, biological function majority Mtb genes unknown. This has revealed need an approach convert raw genome sequence data into information. In past decade, yeast two-hybrid system (Y2H) contributed significantly towards studying virulence and persistence, but several drawbacks. Recently, mycobacterial protein–protein interaction (PPI) technologies have been reported that helped propose functions unknown proteins through “guilt by association” will be discussed this chapter. We examine advantages, disadvantages limitations these systems how can used dissect ­signaling, drug resistance, pathways. also discuss ­mycobacterial PPI exploited force interact discovery small-molecule inhibitors against protein complexes. sum, characterizing PPIs on a genomic scale, it possible assemble ­physiologically relevant pathways mycobacteria, outcome which invaluable determining mechanisms previously uncharacterized proteins.