Ruthenium(II)/Benzonitrile Complex Induces Cytotoxic Effect in Sarcoma-180 Cells by Caspase-Mediated and Tp53/p21-Mediated Apoptosis, with Moderate Brine Shrimp Toxicity.
作者： Raquel Santos Faria , Hugo Delleon Silva , Francyelli Mello-Andrade , Wanessa Carvalho Pires , Flávia de Castro Pereira
DOI: 10.1007/S12011-020-02098-8
关键词:
摘要: Ruthenium(II)/benzonitrile complexes have demonstrated promising anticancer properties. Considering that there are no specific therapies for treating sarcoma, we decided to evaluate the cytotoxic, genotoxic, and lethal effects of cis-[RuCl(BzCN)(phen)(dppb)]PF6 (BzCN = benzonitrile; phen 1,10-phenanthroline; dppb 1,4-bis-(diphenylphosphino)butane), as well mechanism cell death induction occurs against murine sarcoma-180 tumor. Thus, MTT assay was applied assess ruthenium cytotoxicity, showing compound is a more potent inhibitor tumor viability than normal cells (lymphocytes). The comet indicated low genotoxic cells. also showed moderate lethality in Artemia salina. complex induced cycle arrest G0/G1 phase In addition, caused S180 die by apoptosis an increase Annexin-V-positive morphological changes typical apoptotic Additionally, increased gene expression Bax, Casp3, Tp53 By using western blot, observed protein level TNF-R2, p21. conclusion, active selective cells, leading at through caspases-mediated Tp53/p21-mediated pathway.
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sci-hub.se 参考文章(55)
Seunghyi Kook, Vsevolod V. Gurevich, Eugenia V. Gurevich, Arrestins in apoptosis. Handbook of experimental pharmacology. ,vol. 219, pp. 309- 339 ,(2014) , 10.1007/978-3-642-41199-1_16
Flávia de C Pereira, Benedicto AV Lima, Aliny P de Lima, Wanessa C Pires, Thallita Monteiro, Lorena F Magalhães, Wanderson Costa, Angélica E Graminha, Alzir A Batista, Javier Ellena, Elisangela de P Siveira-Lacerda, None, Cis-[RuCl(BzCN)(N-N)(P-P)]PF6 complexes: Synthesis and in vitro antitumor activity: (BzCN=benzonitrile; N-N=2,2'-bipyridine; 1,10-phenanthroline; P-P=1,4-bis(diphenylphosphino) butane, 1,2-bis(diphenylphosphino)ethane, or 1,1'-(diphenylphosphino)ferrocene). Journal of Inorganic Biochemistry. ,vol. 149, pp. 91- 101 ,(2015) , 10.1016/J.JINORGBIO.2015.03.011
Georg Süss-Fink, Arene ruthenium complexes as anticancer agents Dalton Transactions. ,vol. 39, pp. 1673- 1688 ,(2010) , 10.1039/B916860P
Hugh J.M Brady, Gabriel Gil-Gómez, Molecules in focus Bax. The pro-apoptotic Bcl-2 family member, Bax The International Journal of Biochemistry & Cell Biology. ,vol. 30, pp. 647- 650 ,(1998) , 10.1016/S1357-2725(98)00006-5
Linlin Li, Yum-Shing Wong, Tianfeng Chen, Cundong Fan, Wenjie Zheng, Ruthenium complexes containing bis-benzimidazole derivatives as a new class of apoptosis inducers Dalton Transactions. ,vol. 41, pp. 1138- 1141 ,(2012) , 10.1039/C1DT11950H
Jin-Quan Wang, Ping-Yu Zhang, Chen Qian, Xiao-Juan Hou, Liang-Nian Ji, Hui Chao, Mitochondria are the primary target in the induction of apoptosis by chiral ruthenium(II) polypyridyl complexes in cancer cells Journal of Biological Inorganic Chemistry. ,vol. 19, pp. 335- 348 ,(2014) , 10.1007/S00775-013-1069-2
Elisângela de Paula Silveira-Lacerda, Cesar Augusto Sam Tiago Vilanova-Costa, Amélia Hamaguchi, Luiz Alfredo Pavanin, Luiz Ricardo Goulart, Maria Inês Homsi-Brandenburgo, Wagner Batista Dos Santos, Andreimar Martins Soares, Auro Nomizo, None, The Ruthenium Complex cis -(Dichloro)tetraammineruthenium(III) Chloride Presents Selective Cytotoxicity Against Murine B Cell Lymphoma (A-20), Murine Ascitic Sarcoma 180 (S-180), Human Breast Adenocarcinoma (SK-BR-3), and Human T Cell Leukemia (Jurkat) Tumor Cell Lines Biological Trace Element Research. ,vol. 135, pp. 98- 111 ,(2010) , 10.1007/S12011-009-8498-3
C. Gaiddon, P. Jeannequin, P. Bischoff, M. Pfeffer, C. Sirlin, J. P. Loeffler, Ruthenium (II)-Derived Organometallic Compounds Induce Cytostatic and Cytotoxic Effects on Mammalian Cancer Cell Lines through p53-Dependent and p53-Independent Mechanisms Journal of Pharmacology and Experimental Therapeutics. ,vol. 315, pp. 1403- 1411 ,(2005) , 10.1124/JPET.105.089342
S. Fruehauf, M.R. Veldwijk, S. Berlinghoff, N. Basara, C. Baum, M. Flasshove, S. Hegewisch-Becker, N. Kröger, T. Licht, T. Moritz, U.R. Hengge, W. Jens Zeller, S. Laufs, Gene Therapy for Sarcoma Cells Tissues Organs. ,vol. 172, pp. 133- 144 ,(2002) , 10.1159/000065614
Syarifah Nur Syed Abdul Rahman, Norhanom Abdul Wahab, Sri Nurestri Abd Malek, In Vitro Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of Curcuma zedoaria. Evidence-based Complementary and Alternative Medicine. ,vol. 2013, pp. 257108- 257108 ,(2013) , 10.1155/2013/257108