Gastrointestinal phenotype of GAD67lacZ transgenic mice with early postnatal lethality.

摘要: Summary. It has been proposed that g-aminobutyric acid (GABA) in the gut may function as a neurotransmitter, hormone and/or paracrine agent. Our aim was to examine transgenic mice of GAD67-lacZ line with impaired postnatal growth and early lethality for gastrointestinal abnormalities. The tract dissected processed histology, immunohistochemistry, electron microscopy, western blotting measurement GAD activity. Homozygous both sexes displayed an intestinal phenotype characterized by fragile haemorrhagic wall, reduced number villi, epithelial lesions occasional appearance pseudostratified epithelium. GABA-immunoreactive enteroendocrine cells mucin-secreting goblet increased significantly relative wild-type GABA-immunopositive neuronal perikarya lack varicose fibres were observed enteric plexuses mice. Tissue homogenates showed higher levels expression GAD67 GAD65 compared results suggest possible reason underlying impairment is functional GABAergic neurons disintegration Histol Histopathol 20, 75-82 (2005)