p53 alterations in human squamous cell carcinomas and carcinoma cell lines.

摘要: p53 alterations were studied in a group of 22 primary squamous cell carcinomas (SCC) the head and neck 10 lines derived from SCC. Positive immunohistochemical detection was accomplished tumors 7 SCC lines. Loss heterozygosity chromosome 17p, gene is localized, seen five seven studied. DNA sequencing these that had lost one allele showed mutations remaining allele. In addition, six exhibited loss three missense gene. The scattered midregion gene, affecting codons 151, 155, 174, 194, 220, 248, 273. Five modified guanine residues, phenomenon has been associated with effect carcinogens contained tobacco smoke. Collectively data show approximately 50% abnormalities it interest to note most invasive lines, as determined an vivo assay using xenotransplantation tumor cells into denuded rat tracheal grafts, intense staining. Similarly, very advanced tumors, four immunostain. These observations support evidence this suppressor could be related late events progression.