A Preferentially Segregated Recycling Vesicle Pool of Limited Size Supports Neurotransmission in Native Central Synapses

摘要: At small central synapses, efficient turnover of vesicles is crucial for stimulus-driven transmission, but how the structure this recycling pool relates to its functional role remains unclear. Here we characterize organizational principles at native hippocampal synapses with nanoscale resolution using fluorescent dye labeling and electron microscopy. We show that broadly scales magnitude total vesicle pool, average size (∼45 vesicles), highly variable, regulated by CDK5/calcineurin activity. Spatial analysis demonstrates are preferentially arranged near active zone segregation abolished actin stabilization, slowing rate activity-driven exocytosis. Our approach reveals a similarly biased distribution in visual cortex activated sensory stimulation in vivo. suggest release of a limited relies on their favored spatial positioning within terminal.