Enzymatic Hydrolysis and Simultaneous Extraction for Preparation of Genipin from Bark of Eucommia ulmoides after Ultrasound, Microwave Pretreatment

作者: Lili Li , Yupin Guo , Lianfei Zhao , Yuangang Zu , Huiyan Gu

DOI: 10.3390/MOLECULES201018717

关键词:

摘要: A continuous process based on the combination of ultrasounds and/or microwaves pretreatments followed by enzymatic hydrolysis and simultaneous extraction (EHSE) has been proposed to recover genipin from Eucommia ulmoides bark. At first, in pretreatment step, mixture 1.0 g dried bark powder 10 mL deionized water were irradiated microwave under 500 W for min. Then, optimal conditions as follows: 0.5 mg/mL cellulase concentration, 4.0 pH enzyme solution, 24 h incubation time 40 °C temperature. After incubation, ethanol was added extract 30 min ultrasound. EHSE treatment, yield could reach 1.71 μmol/g. Moreover, scanning electron micrographs illustrated that severe structural disruption plant obtained EHSE. The results indicated method provided a good alternative preparation well other herbs.

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