t10,c12 conjugated linoleic acid upregulates hepatic de novo lipogenesis and triglyceride synthesis via mTOR pathway activation.

摘要: In mice, supplementation of t10,c12 conjugated linoleic acid (CLA) increases liver mass and hepatic steatosis via increasing uptake fatty acids released from adipose tissues. However, the effects CLA on lipid synthesis associated mechanisms are largely unknown. Thus, we tested hypothesis that gut microbiota-producing would induce de novo lipogenesis triglyceride (TG) in HepG2 cells, promoting accumulation. It was found treatment with (100 micrometer) for 72 h increased neutral accumulation enhanced incorporation acetate, palmitate, oleate, 2- deoxyglucose into TG. Furthermore, led to mRNA expression protein levels lipogenic genes including SREBP1, ACC1, FASN, ELOVL6, GPAT1, DGAT1, presenting potential by which may increase deposition. Most strikingly, 3 phosphorylation mTOR, S6K, S6. Taken together, activates TG through activation mTOR/SREBP1 pathway, consequent cells.