Human papillomavirus DNA in respiratory papillomatosis detected by in situ hybridization and the polymerase chain reaction.
作者： H Torloni , L L Villa , J E Levi , V N Alberti , R Delcelo
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摘要: The authors have demonstrated the presence of human papillomavirus (HPV) types 6 and 11 in 10 13 (77%) juvenile laryngeal papillomatosis by situ DNA hybridization using as probes radiolabeled DNAs HPVs 6, 11, 16, 18. Of six specimens from adult assayed same technique, only 33% were positive. Immunohistochemistry to detect HPV capsid antigens performed on serial sections gave positive signals 44% (8 18) specimens, all lesions. These results agreement with hybridization, except two cases. When both series (juvenile adult) analysed amplification a 450-bp fragment corresponding L1 ORF genomes directed polymerase chain reaction, frequency rose 100%. Our data agree concept that is implicated etiology papillomatosis.
参考文章(15)
J. Gupta, K. Shah, S. Pilotti, F. Rilke, Association of human papillomavirus type 16 with neoplastic lesions of the vulva and other genital sites by in situ hybridization. American Journal of Pathology. ,vol. 127, pp. 206- 215 ,(1987)
Allan L. Abramson, Janet L. Brandsma, Bettie M. Steinberg, Barbara Winkler, Presence of Human Papillomavirus Type 16 Related Sequences in Verrucous Carcinoma of the Larynx Cancer Research. ,vol. 46, pp. 2185- 2188 ,(1986)
J Burns, A K Graham, C Frank, K A Fleming, M F Evans, J O McGee, Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by non-isotopic in situ hybridisation. Journal of Clinical Pathology. ,vol. 40, pp. 858- 864 ,(1987) , 10.1136/JCP.40.8.858
Janet C. Byrne, Ming-Sound Tsao, Richard S. Fraser, Peter M. Howley, Human Papillomavirus-11 DNA in a Patient with Chronic Laryngotracheobronchial Papillomatosis and Metastatic Squamous-Cell Carcinoma of the Lung The New England Journal of Medicine. ,vol. 317, pp. 873- 878 ,(1987) , 10.1056/NEJM198710013171406
D K Shibata, N Arnheim, W J Martin, Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. Journal of Experimental Medicine. ,vol. 167, pp. 225- 230 ,(1988) , 10.1084/JEM.167.1.225
P. Ward, P. Mounts, Transcriptional Activity of Human Papillomavirus Type 6 in Respiratory Tract Papillomata Journal of General Virology. ,vol. 69, pp. 1671- 1682 ,(1988) , 10.1099/0022-1317-69-7-1671
L. Gissmann, L. Wolnik, H. Ikenberg, U. Koldovsky, H. G. Schnurch, H. zur Hausen, Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers Proceedings of the National Academy of Sciences of the United States of America. ,vol. 80, pp. 560- 563 ,(1983) , 10.1073/PNAS.80.2.560
HASKINS KASHIMA, TZYY-CHOOU WU, DENNIS HEFFNER, VINCENT HYAMS, PHOEBE MOUNTS, Baltimore, Carcinoma ex-papilloma: Histologic and virologic studies in whole-organ sections of the larynx Laryngoscope. ,vol. 98, pp. 619- 624 ,(1988) , 10.1288/00005537-198806000-00008
Ernst L. Wynder, Lirio S. Covey, Kiyóhiko Mabuchi, Margaret Mushinski, Environmental factors in cancer of the larynx.A second look Cancer. ,vol. 38, pp. 1591- 1601 ,(1976) , 10.1002/1097-0142(197610)38:4<1591::AID-CNCR2820380425>3.0.CO;2-R
P. Mounts, K. V. Shah, H. Kashima, Viral etiology of juvenile- and adult-onset squamous papilloma of the larynx. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 5425- 5429 ,(1982) , 10.1073/PNAS.79.17.5425