Enhanced production of proteinaceous materials in eucaryotic cells

摘要: Disclosed is a method of enhancing expression recombinant DNA in eucaryotic cells. A tissue specific enhancer element obtained from the genome an organism and active selected type combined with transcriptionally competent transcription unit comprising promoter exons encoding for proteinaceous material interest (or its precursor). This transfected into cells derived same as which normally functions to enhance endogeneous DNA. The resulting transformants express at high levels increases copy number mRNA. operates increase independent orientation position provided it located within region on DNA, generally between about 1-10 kilobases (kb) 3' or 5' end unit.