Development of a Diagnostic Test Based on the Polymerase Chain Reaction (PCR) to Identify Strains of R. solanacearum Exhibiting the Biovar 2 Genotype
作者： M. Fegan , G. Holoway , A. C. Hayward , J. Timmis
DOI: 10.1007/978-3-662-03592-4_5
关键词:
摘要: R. solanacearum has been classified into three races based on host range (Buddenhagen et al. 1962) and five biovars oxidation of sugars sugar alcohols (Hayward 1964) the only agreement between two schemes is that biovar 2 equivalent to race 3. 2/race 3 isolates have a very narrow range, being limited almost entirely potatoes. Bacterial wilt (Brown rot) caused by significant disease potatoes world wide. Infection potato tubers with may become latent under conducive environmental conditions. Other can infect but it phenotype most persistent potentially destructive for Consequently, there need rapid, sensitive diagnostic tests identification plant material infected phenotype. Tests detection in traditionally directed against entire species not concentrated Skoglund (1993) report molecular immunological techniques applied Burundi, all were toward 2/ The test described identify 3/biovar DNA probe Cook Sequeira (1991). We describe here first employing polymerase chain reaction.
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