A freeze-squeeze method for recovering long DNA from agarose gels.
作者： R.W.J. Thuring , J.P.M. Sanders , P. Borst
DOI: 10.1016/0003-2697(75)90739-3
关键词:
摘要: Long DNA can be recovered from agarose gels after electrophoresis by freezing the gel slices and manually squeezing out liquid containing DNA. With this method recoveries of phage T7 (molecular weight 25 × 106) open closed forms circular PM2 6 were about 70%. Sedimentation analysis shows that extruded has not sustained double- or single-stranded breaks. The used without further purification as substrate for restriction endonuclease HindII,III, Hemophilus influenzae, DNA·DNA hybridization electron microscopy.
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