Isolation and localization of DNA segments

作者: James F. Gusella , David Housman , Aviva Varsanyi-Breiner , Cheryl Keys , Theodore T. Puck

DOI:

关键词: DNADeletion mutantChromosomeBiologyCell hybridsGenetics

摘要: A process of isolating and identifying cloned DNA segments from defined regions a chromosome by preparing series cell hybrids first species second species, which contain or deletion mutant as the o The Government has rights in this invention pursuant to Contract Number CA-5-14051 awarded Department Health Human Services, PCM 77-17747 National Science Foundation, 1-713 Foundation-March Dimes.

参考文章(13)
J. Gusella, A. Varsanyi-Breiner, F. T. Kao, C. Jones, T. T. Puck, C. Keys, S. Orkin, D. Housman, Precise localization of human beta-globin gene complex on chromosome 11 Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 5239- 5242 ,(1979) , 10.1073/PNAS.76.10.5239
R. J. Britten, D. E. Kohne, Repeated Sequences in DNA Science. ,vol. 161, pp. 529- 540 ,(1968) , 10.1126/SCIENCE.161.3841.529
Aviva Varsanyi-Breiner, James F. Gusella, Cheryl Keys, David E. Housman, Daniel Sullivan, Normand Brisson, Desh Pal S. Verma, The organization of a nuclear DNA sequence from a higher plant: molecular cloning and characterization of soybean ribosomal DNA. Gene. ,vol. 7, pp. 317- 334 ,(1979) , 10.1016/0378-1119(79)90051-9
F. T. Kao, C. Jones, T. T. Puck, Genetics of somatic mammalian cells: genetic, immunologic, and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 73, pp. 193- 197 ,(1976) , 10.1073/PNAS.73.1.193
F. Blattner, B. Williams, A. Blechl, K Denniston-Thompson, H. Faber, L Furlong, D. Grunwald, D. Kiefer, D. Moore, J. Schumm, E. Sheldon, O Smithies, Charon phages: safer derivatives of bacteriophage lambda for DNA cloning Science. ,vol. 196, pp. 161- 169 ,(1977) , 10.1126/SCIENCE.847462
F. Blattner, A. Blechl, K Denniston-Thompson, H. Faber, J. Richards, J. Slightom, P. Tucker, O Smithies, Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections. Science. ,vol. 202, pp. 1279- 1284 ,(1978) , 10.1126/SCIENCE.725603
E. Davidson, R. Britten, Regulation of gene expression: possible role of repetitive sequences Science. ,vol. 204, pp. 1052- 1059 ,(1979) , 10.1126/SCIENCE.451548
R.W.J. Thuring, J.P.M. Sanders, P. Borst, A freeze-squeeze method for recovering long DNA from agarose gels. Analytical Biochemistry. ,vol. 66, pp. 213- 220 ,(1975) , 10.1016/0003-2697(75)90739-3