Proteomic analysis of the effects of cell culture density on the metastasis of breast cancer cells.

摘要: Cancer cell progression and proliferation increase density, resulting in changes to the tumour site, including microenvironment. What is not known if increased density influences aggressiveness of cancer cells, especially their proliferation, migration, invasion capabilities. In this study, we found that dense culture enhances metastatic lines, 4T1 ZR-75-30, by increasing However, a less line, MCF-7, did show an aggressiveness, following conditions. We conducted differential proteomic analysis on cells cultured under or sparse conditions identified expression for proteins involved focal adhesion, cytoskeletal reorganization, transendothelial migration. contrast, grown had higher levels metabolism, lipid phospholipid binding, cholesterol transporter activity, protein binding. These results suggest high-density microenvironment can cause change cellular behaviour, leading towards more aggressive cancers. SIGNIFICANCE OF THE STUDY: Metastasis obstacle clinical treatment cancer. cultures made potent terms invasion. The bioinformatic analyses provided some valuable clues further intensive studies about effects which were associated with events such as pre-mRNA splicing RNA transport, adhesion cytoskeleton ribosome biogenesis, proteins, JAM-1 S100A11. This investigation gives us new perspectives investigate metastasis mechanisms related sites.