Consolidated bioprocessing of highly concentrated Jerusalem artichoke tubers for simultaneous saccharification and ethanol fermentation.

摘要: Consolidated bioprocessing (CBP) of Jerusalem artichoke tuber (Jat) for ethanol production is one the most promising options an alternate biofuel technology development. The technical barriers include weak saccharolytic enzyme (inulinase) activity fermentation strain, and well mixing high viscous slurry at highly concentrated Jat loading. In this study, Saccharomyces cerevisiae DQ1 was found to produce relatively large amount inulinase hydrolysis inulin in Jat, helical ribbon stirring bioreactor used provided performance under Even a loading up 35% (w/w) CBP allowed. results obtained from study have demonstrated feasibility developing process yield 128.7 g/L theoretical 73.5%, respectively. This level higher than others reported so far. could provide practical economically sustainable using containing biomass feedstock such as least 35%. Biotechnol. Bioeng. 2013;110: 2606–2615. © 2013 Wiley Periodicals, Inc.