Inactivation of Mycobacterium bovis ssp. caprae in high-temperature, short-term pasteurized pilot-plant milk

摘要: Experiments to determine the efficacy of high temperature, short time (HTST) pasteurization milk in terms inactivation pathogenic microorganisms were mainly performed between 1930 and 1960. Among target organisms Mycobacterium bovis tuberculosis. As a result, Codex Alimentarius prescribes that HTST treatment should lead significant reduction during pasteurization. Due development improved methods for detection survivors more advanced heating technology, verification this requirement seemed be necessary. To address recent outbreaks tuberculosis cattle caused by M. ssp. caprae (M. caprae) southern regions Germany, organism was tested compared with bovis). pilot plant 3 strains 1 strain bovis. In preliminary trials at fixed holding 25 s, temperature which occurred 62.5°C all strains. D-values (decimal times) kinetics, 65, 62.5, 60°C times 16.5, 25, 35 s. At 65°C, ranged from 6.8 7.8 62.5°C, 14.5 18.1 Low observed 60°C. When low slope curve allowed calculation D-value, these 40.8 129.9 log10 reductions, highest values 4.1 4.9 log The showed similar resistance heat. Standard result thus requirements pathogens process are far exceeded.