Early Transcriptome Response of Lactococcus lactis to Environmental Stresses Reveals Differentially Expressed Small Regulatory RNAs and tRNAs

摘要: Bacteria can deploy various mechanisms to combat environmental stresses. Many genes have previously been identified in Lactococcus lactis that are involved sensing the stressors and those regulating mounting a defense against stressful conditions. However, expression of small regulatory RNAs (sRNAs) during industrially relevant stress conditions has not assessed yet L. lactis, while sRNAs shown be many responses other bacteria. We reported presence hundreds putative used high-throughput RNA sequencing (RNA-seq) this study assess their under six different The uniformly designed experimental set-up enabled highly reliable comparison between revealed differentially expressed applied. primary NCDO712 was benchmarked earlier work and, for first time, differential transfer (tRNAs) from recently sequenced plasmids NCDO712. Although, we only applied stresses 5 min, majority well-known specific stress-induced already expressed. find most tRNAs decrease after all applied, except number, which increased upon cold stress. Starvation induce highest response, both terms number level genes. Our data pinpoints novel stress-related uncharacterized sRNAs, calls further assessment molecular cellular function. These insights furthermore could impact way parameters bacterial culture production milk fermentation, as very short greatly alter gene expression.