Method of screening candidate compounds for susceptibility to biliary excretion

摘要: A method of screening a candidate compound for susceptibility to biliary excretion by hepatocyte transport protein. The includes the steps providing culture hepatocytes comprising protein, having at least one bile canaliculus; exposing culture; and determining an amount in canaliculus, canaliculus indicating In some embodiments comprises inhibiting expression measuring comparing amounts canalicules with without inhibition difference indicates embodiment, protein is inhibited through introduction RNA sequence corresponding coding strand gene encoding into hepatocyte. Optionally, long-term sandwich configuration. particularly applicable multiple compounds single effort.