2,3-Dihydroxybenzoate 3,4-oxygenase from Pseudomonas fluorescens—Oxidation of a substrate analog

摘要: 2,3-Dihydroxybenzoate is oxidized by extracts of Pseudomonas fluorescens to α-hydroxymuconic semialdehyde and CO2. A noninducing substrate analog, 2,3-dihydroxy-p-toluate, has now been used determine the site ring cleavage. 2,3-Dihydroxy-p-toluate quantitatively with consumption 1 mole O2, evolution CO2, accumulation 2,6-diketoheptenoate. The product was characterized (1) spectral analysis isolated acid, (2) closure in presence NH4+ form 6-methylpicolinate, (3) its ready conversion acetate 76% yield, Ps. aeruginosa T1. It concluded that enzymic cleavage benzenoid nucleus this oxygenase between carbon atoms 3 4, enzyme named 2,3-dihydroxybenzoate 3,4-oxygenase.