Clearing muddied waters: Capture of environmental DNA from turbid waters.

摘要: Understanding the differences in efficiencies of various methods to concentrate, extract, and amplify environmental DNA (eDNA) is vital for best performance eDNA detection. Aquatic systems vary characteristics such as turbidity, concentration, inhibitor load, thus affecting capture efficiency. Application techniques detection terrestrial invasive or endangered species may require sampling at intermittent water sources that are used drinking cooling; these bodies often be stagnant turbid. We present our practices technique wild pig samples, a protocol will have wide applicability elusive vertebrate species. determined practice turbid system was concentrate from 15 mL sample via centrifugation, purify with DNeasy mericon Food kit, remove inhibitors Zymo Inhibitor Removal Technology columns. Further, we compared sensitivity conventional PCR quantitative found more sensitive detecting lower concentrations eDNA. show significant among each step capture, emphasizing importance optimizing interest.