Killer artificial antigen-presenting cells: a novel strategy to delete specific T cells

摘要: Several cell-based immunotherapy strategies have been developed to specifically modulate T cell–mediated immune responses. These methods frequently rely on the utilization of tolerogenic cell–based antigen-presenting cells (APCs). However, APCs are highly sensitive cytotoxic T-cell responses, thus limiting their therapeutic capacity. Here, we describe a novel bead-based approach responses in an antigen-specific fashion. We generated killer artificial (κaAPCs) by coupling apoptosis-inducing α-Fas (CD95) IgM mAb together with HLA-A2 Ig molecules onto beads. κaAPCs deplete targeted Fas/Fas ligand (FasL)–dependent depletion cocultures is rapidly initiated (30 minutes), dependent amount and independent activation-induced cell death (AICD). represent technology that can control therefore has potential for use treatment autoimmune diseases allograft rejection.