Antigen-Specific Killer Polylactic-Co-Glycolic Acid (PLGA) Microspheres Can Prolong Alloskin Graft Survival in a Murine Model
作者： Wei Wang , Kun Fang , Xiaobing Wang , Miaochen Li , You Wu
DOI: 10.3109/08820139.2015.1014098
关键词:
摘要: Background: The strategy of specifically depleting antigen-specific T cells can potentially be used for the treatment allograft rejection and autoimmunity because it does not suppress overall immune systems.Methods: In this study, we generated killer polylactic-co-glycolic acid (PLGA) microspheres by covalently coupling major histocompatibility complex (MHC) class I antigens apoptosis-inducing anti-Fas monoclonal antibody (mAb) onto PLGA microspheres. A modified double-emulsion method was preparation cell-sized H-2Kb/peptide monomers were in-house analyzed through flow cytometry. administered intravenously into BALB/c mice (H-2Kd) that had previously been grafted with skin squares from C57BL/6 (H-2Kb). Tumor cell challenge third-party mixed lymphocyte culture to assess general functions host.Results: alloskin graft survival prolonged 4 days. microspheres...
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