Dendritic cells overexpressing CD95 (Fas) ligand elicit vigorous allospecific T-cell responses in vivo.

摘要: Dendritic cells (DCs) genetically engineered to overexpress CD95 (Fas) ligand (CD95L-DC) were proposed as tools induce peripheral tolerance alloantigens. Herein, we observed that CD95L-DC obtained after retroviral gene transfer in bone marrow (BM) precursors derived from CD95-deficient (lpr/lpr) mice elicit much stronger allospecific type 1 helper T-cell and cytotoxic activities than control DCs upon injection vivo, although they lower responses vitro. Indeed, a single of prepared C57BL/6 was sufficient prime bm13 recipients for acute rejection skin allografts otherwise tolerated the context this weak major histocompatibility complex (MHC) class I incompatibility. Massive neutrophil infiltrates depending on interleukin (IL)–1 signaling at sites injection. Experiments IL-1 receptor–deficient or animals injected with depleting anti-Gr1 monoclonal antibody (mAb) established recruitment is required development vigorous vivo.