The lipase from Y. lipolytica : genetics, production, regulation and biochemical characterisation
作者： Patrick Fickers , Vincent Sauveplane , Jean-Marc Nicaud
DOI: 10.1007/978-3-642-38583-4_3
关键词:
摘要: The yeast Yarrowia lipolytica possesses multiple paralogues of genes dedicated to hydrophobic substrates metabolization. Among them, 16 lipase encoding genes, involved in lipid or grease breakdown, were highlighted the genome. However, little information on all those has been yet obtained. Microarray data suggest that only a few them could be expressed. Lipase synthesis seems dependent fatty acid oil used as carbon source confirming high adaptation Y. different substrate. This review focuses biochemical characterization enzymes with special emphasis Lip2p which is isoenzyme mainly synthesized by lipolytica. 3D structure this established homology modeling confirms sensu stricto lid covering active site enzyme its closed conformation. Recent findings conditioning dehydrated liquid formulation and immobilization entrapment natural polymers from either organic mineral origins are also discussed together long-term storage strategies.
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