A stable lipase from Candida lipolytica: cultivation conditions and crude enzyme characteristics.
作者： Fatima Ventura Pereira-Meirelles , Maria Helena Miguez Rocha-LeãO , Geraldo Lippel Santť Anna
DOI: 10.1007/BF02920414
关键词: Lipase 、 Triolein 、 Oleic acid 、 Enzyme 、 Catabolite repression 、 Substrate (chemistry) 、 Biochemistry 、 Biology 、 Triacylglycerol lipase 、 Yeast
摘要: Although lipases have been intensively studied, some aspects of enzyme production like substrate uptake, catabolite repression, and stability under long storage periods are seldom discussed in the literature. This work deals with lipase by a new selected strain Candida lipolytica. Concerning nutrition, it was observed that inorganic nitrogen sources were not as effective peptone, oleic acid or triacylglycerides (TAG) essential carbon sources. Repression glucose stimulation chain TAG (triolein olive oil) observed. Extracellular activity only at high levels late stationary phase, whereas intracellular constant almost undetectable during cultivation period, suggesting produced attached to cell wall, mainly beginning cultivation. The crude this yeast shows following optima conditions: pH 8.0-10.0, temperature 55°C. Moreover, preparation maintains its full for least 370 d 5°C.
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