Yeast Two Hybrid Analyses Reveal Novel Binary Interactions between Human Cytomegalovirus-Encoded Virion Proteins

摘要: Human cytomegalovirus (HCMV) is the largest human herpesvirus and its virion contains many viral encoded proteins found in capsid, tegument, envelope. In this study, we carried out a yeast two-hybrid (YTH) analysis to study potential binary interactions among 56 HCMV-encoded proteins. We have tested more than 3,500 pairwise combinations for YTH analysis, identified 79 that involve 37 Forty five of were also cells expressing by co-immunoprecipitation (co-IP) experiments. To our knowledge, 58 revealed including those 24 co-IP experiments, not been reported before. Novel between capsid tegument proteins, envelope Furthermore, both experiments 9, 7, 5 involved with UL25, UL24, UL89, respectively, suggesting these “hub” may function as organizing centers connecting multiple mature recruiting other during maturation assembly. Our provides framework HCMV investigate roles protein-protein formation or process.