High Throughput Screening of Natural Products for Anti-mitotic Effects in MDA-MB-231 Human Breast Carcinoma Cells

摘要: Some of the most effective anti-mitotic microtubule-binding agents, such as paclitaxel (Taxus brevifolia) were originally discovered through robust NCI botanical screenings. In this study, a high-through microarray format was utilized to screen 897 aqueous extracts commonly used natural products (0.00015–0.5 mg/ml) relative for effects (independent toxicity) on proliferation MDA-MB-231 cells. The data obtained showed that less than 1.34 % tested inhibitory growth (IG50) properties <0.0183 mg/ml. potent anti-mitotics Mandrake root (Podophyllum peltatum), Truja Twigs (Thuja occidentalis), Colorado desert mistletoe (Phoradendron flavescens), Tou Gu Cao Speranskia Herb (Speranskia tuberculata), Bentonite Clay, Bunge Root (Pulsatilla chinensis), Brucea Fruit (Brucea javanica), Madder (Rubia tinctorum), Gallnut Chinese Sumac (Melaphis Elecampane (Inula Helenium), Yuan Zhi (Polygala tenuifolia), Pagoda Tree (Melia Toosendan), Stone (Collinsonia Canadensis) and others American Witchhazel, Arjun Bladderwrack. strongest tumoricidal herbs identified from amongst subset evaluated wild yam (Dioscorea villosa), beth-root (Trillium Pendulum) alkanet-root (Lithospermum canescens). Additional lesser-recognized herb: tuberculata) which inhibition BT-474 (human ductal breast carcinoma) Ishikawa endometrial adenocarcinoma) cells with ability block replicative DNA synthesis leading G2 arrest in conclusion, these findings present potency resources against human carcinoma cell division.