Two-dimensional nanosecond electric field mapping based on cell electropermeabilization.
作者： Meng-Tse Chen , Chunqi Jiang , P Thomas Vernier , Yu-Hsuan Wu , Martin A Gundersen
关键词:
摘要: Nanosecond, megavolt-per-meter electric pulses cause permeabilization of cells to small molecules, programmed cell death (apoptosis) in tumor cells, and are under evaluation as a treatment for skin cancer. We use nanoelectroporation fluorescence imaging construct two-dimensional maps the field associated with delivery 15 ns, 10 kV monolayers human prostate cancer line PC3 from three different electrode configurations: single-needle, five-needle, flat-cut coaxial cable. Influx normally impermeant fluorescent dye YO-PRO-1 serves sensitive indicator membrane permeabilization. The level emission after pulse exposure is proportional applied strength. Spatial distributions were compared plane normal center axis 15-20 μm tip electrode. Measurement results agree well models arrangements evaluated this study. This live-cell method measuring nanosecond pulsed distribution provides an operationally meaningful calibration designs biological applications permits visualization relative sensitivities types nanoelectropulse stimulation. PACS Codes: 87.85.M-
biomedcentral.com
springer.com
core.ac.uk
physmathcentral.com参考文章(19)
P Thomas Vernier, Yinghua Sun, Martin A Gundersen, Nanoelectropulse-driven membrane perturbation and small molecule permeabilization BMC Cell Biology. ,vol. 7, pp. 37- 37 ,(2006) , 10.1186/1471-2121-7-37
W.M. Arnold, G. Fuhr, Increasing the permittivity and conductivity of cellular electromanipulation media ieee industry applications society annual meeting. ,vol. 2, pp. 1470- 1476 ,(1994) , 10.1109/IAS.1994.377619
K.H. Schoenbach, R.P. Joshi, J.F. Kolb, Nianyong Chen, M. Stacey, P.F. Blackmore, E.S. Buescher, S.J. Beebe, Ultrashort electrical pulses open a new gateway into biological cells Proceedings of the IEEE. ,vol. 92, pp. 1122- 1137 ,(2004) , 10.1109/JPROC.2004.829009
D. Haemmerich, P. F. Laeseke, Thermal tumour ablation: Devices, clinical applications and future directions International Journal of Hyperthermia. ,vol. 21, pp. 755- 760 ,(2005) , 10.1080/02656730500226423
Damijan Miklavčič, Katarina Beravs, Dejan Šemrov, Maja Čemažar, Franci Demšar, Gregor Serša, The Importance of Electric Field Distribution for Effective in Vivo Electroporation of Tissues Biophysical Journal. ,vol. 74, pp. 2152- 2158 ,(1998) , 10.1016/S0006-3495(98)77924-X
Edward B. Garon, David Sawcer, P. Thomas Vernier, Tao Tang, Yinghua Sun, Laura Marcu, Martin A. Gundersen, H. Phillip Koeffler, In vitro andin vivo evaluation and a case report of intense nanosecond pulsed electric field as a local therapy for human malignancies International Journal of Cancer. ,vol. 121, pp. 675- 682 ,(2007) , 10.1002/IJC.22723
Richard Nuccitelli, Uwe Pliquett, Xinhua Chen, Wentia Ford, R. James Swanson, Stephen J. Beebe, Juergen F. Kolb, Karl H. Schoenbach, Nanosecond pulsed electric fields cause melanomas to self-destruct Biochemical and Biophysical Research Communications. ,vol. 343, pp. 351- 360 ,(2006) , 10.1016/J.BBRC.2006.02.181
P.Thomas Vernier, Yinghua Sun, Laura Marcu, Sarah Salemi, Cheryl M Craft, Martin A Gundersen, Calcium bursts induced by nanosecond electric pulses. Biochemical and Biophysical Research Communications. ,vol. 310, pp. 286- 295 ,(2003) , 10.1016/J.BBRC.2003.08.140
P. Thomas Vernier, Yinghua Sun, Laura Marcu, Cheryl M. Craft, Martin A. Gundersen, Nanoelectropulse-induced phosphatidylserine translocation. Biophysical Journal. ,vol. 86, pp. 4040- 4048 ,(2004) , 10.1529/BIOPHYSJ.103.037945
Tao Tang, Fei Wang, Andras Kuthi, Martin Gundersen, Diode Opening Switch Based Nanosecond High Voltage Pulse Generators for Biological and Medical Applications IEEE Transactions on Dielectrics and Electrical Insulation. ,vol. 14, pp. 878- 883 ,(2007) , 10.1109/TDEI.2007.4286519