Ultrastructural Characterization of the New NG97ht Human-Derived Glioma Cell Line Using Two Different Electron Microscopy Technical Procedures

摘要: On the basis of transmission electron microscopy observations in tumor cell lines, oncologists have made innumerous diagnostic and therapeutical progresses. Following this path, UNICAMP immunopathologies laboratory established NG97 line derived from a human astrocytoma grade III, which when injected to athymic nude mouse flank developed IV astrocytoma. In study, we focused on ultrastructural characterization cells after being recovered xenotransplant (NG97ht). These culture were assayed by two different procedures characterize ultrastructures related astrocytomas observe their structures through subcultivation. Additionally, compara- tive morphological descriptions passages these technical could be useful tool for improving lineage protocols. Results many pas- sage showed similarities, suggest malignant glioblastoma phenotypes. first procedure, NG97ht harvested then incorporated into aga- rose before subjecting them protocols, whereas second one, mono- layer grew cover slides. Comparison among protocols revealed that organelles, cyto- plasmatic extensions, spatial conformation filopodia, attachment substrate more preserved procedure. Furthermore, latter unique ellipsoi- dal structure was observed, already described dealing with gliosarcoma elsewhere. Therefore, analyses demonstrated new using microscopy. Moreover, it has been shown procedure provides detailed information compared first. Microsc. Res. Tech.