Gene silencing by RNA Interference in the white rot fungus Phanerochaete chrysosporium.
作者： Avi Matityahu , Yitzhak Hadar , Carlos G. Dosoretz , Paula A. Belinky
DOI: 10.1128/AEM.02433-07
关键词:
摘要: The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. manganese-containing superoxide dismutase gene (MnSOD1) was used as target for RNAi. plasmid constructed silencing contained a transcriptional unit hairpin expression. Significantly lower MnSOD expression at both mRNA and protein activity levels detected RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies gene, this construct sufficient to decrease enzymatic by much 70% relative control levels. Implementation technique provides an alternative genetic tool studies function, particularly essential genes or families.
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