Hypoxia, reoxygenation and cytosolic manganese superoxide dismutase (cMnSOD) silencing in Litopenaeus vannamei: effects on cMnSOD transcripts, superoxide dismutase activity and superoxide anion production capacity.
作者： Antonio García-Triana , Tania Zenteno-Savín , Alma Beatriz Peregrino-Uriarte , Gloria Yepiz-Plascencia
DOI: 10.1016/J.DCI.2010.06.018
关键词:
摘要: Abstract The effects of silencing the mRNA cytosolic manganese superoxide dismutase (cMnSOD), an enzyme involved in antioxidant defense, were analyzed Whiteleg shrimp, Litopenaeus vannamei adults. Shrimp intramuscularly injected with long dsRNAs corresponding to N-terminal portion cMnSOD and held under normoxic conditions for 24 h. Another group shrimp was exposed hypoxia 6 h followed by reoxygenation 1 h. had lower transcripts gills hepatopancreas. In silenced (SOD) activity decreased but not subjected SOD hepatopancreas; production anion (O 2 − ) hemocytes also this group. Reoxygenation reverted effect increasing levels transcripts, O . These results suggest that contributes significantly hepatopancreas indicate its importance redox system regulation L.
cabdirect.org
elsevier.com
sci-hub.se 参考文章(31)
Shirley Hiu-Kwan Tiu, Siu-Ming Chan, The use of recombinant protein and RNA interference approaches to study the reproductive functions of a gonad-stimulating hormone from the shrimp Metapenaeus ensis. FEBS Journal. ,vol. 274, pp. 4385- 4395 ,(2007) , 10.1111/J.1742-4658.2007.05968.X
Juana M. Lugo, Yuliet Morera, Tania Rodríguez, Alberto Huberman, Laida Ramos, Mario P. Estrada, Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti. Functional analysis by double-stranded RNA interference technique. FEBS Journal. ,vol. 273, pp. 5669- 5677 ,(2006) , 10.1111/J.1742-4658.2006.05555.X
Jerome Ho Lam Hui, Stephen S. Tobe, Siu-Ming Chan, Characterization of the putative farnesoic acid O-methyltransferase (LvFAMeT) cDNA from white shrimp, Litopenaeus vannamei: Evidence for its role in molting Peptides. ,vol. 29, pp. 252- 260 ,(2008) , 10.1016/J.PEPTIDES.2007.08.033
Erchao Li, Liqiao Chen, Ceng Zeng, Na Yu, Zequan Xiong, Xuefen Chen, Jian G. Qin, Comparison of digestive and antioxidant enzymes activities, haemolymph oxyhemocyanin contents and hepatopancreas histology of white shrimp, Litopenaeus vannamei, at various salinities Aquaculture. ,vol. 274, pp. 80- 86 ,(2008) , 10.1016/J.AQUACULTURE.2007.11.001
Avi Matityahu, Yitzhak Hadar, Carlos G. Dosoretz, Paula A. Belinky, Gene silencing by RNA Interference in the white rot fungus Phanerochaete chrysosporium. Applied and Environmental Microbiology. ,vol. 74, pp. 5359- 5365 ,(2008) , 10.1128/AEM.02433-07
Javier Robalino, Thomas Bartlett, Eleanor Shepard, Sarah Prior, Guillermo Jaramillo, Edward Scura, Robert W. Chapman, Paul S. Gross, Craig L. Browdy, Gregory W. Warr, Double-Stranded RNA Induces Sequence-Specific Antiviral Silencing in Addition to Nonspecific Immunity in a Marine Shrimp: Convergence of RNA Interference and Innate Immunity in the Invertebrate Antiviral Response? Journal of Virology. ,vol. 79, pp. 13561- 13571 ,(2005) , 10.1128/JVI.79.21.13561-13571.2005
Tania Zenteno-Savín, Ricardo Saldierna, Mauricio Ahuejote-Sandoval, Superoxide radical production in response to environmental hypoxia in cultured shrimp Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. ,vol. 142, pp. 301- 308 ,(2006) , 10.1016/J.CBPC.2005.11.001
Chang-Che Li, Jiann-Chu Chen, The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus under low and high pH stress. Fish & Shellfish Immunology. ,vol. 25, pp. 701- 709 ,(2008) , 10.1016/J.FSI.2008.01.007
Gracia A. Gómez-Anduro, Rogerio R. Sotelo-Mundo, Adriana Muhlia-Almazán, Gloria Yepiz-Plascencia, Tissue-specific expression and molecular modeling of cytosolic manganese superoxide dismutases from the white shrimp Litopenaeus vannamei Developmental & Comparative Immunology. ,vol. 31, pp. 783- 789 ,(2007) , 10.1016/J.DCI.2006.11.007
Bo Yu, Xiaobin Zhao, L. James Lee, Robert J. Lee, Targeted Delivery Systems for Oligonucleotide Therapeutics Aaps Journal. ,vol. 11, pp. 195- 203 ,(2009) , 10.1208/S12248-009-9096-1