Cyclooxygenase gene expression is down-regulated by heparin-binding (acidic fibroblast) growth factor-1 in human endothelial cells.

摘要: The expression of cyclooxygenase (EC 1.14.99.1, Cox), a rate-limiting enzyme in the biosynthesis inflammatory prostanoids, is regulated by growth factors and cytokines. We have shown previously that cytokine interleukin-1, an inhibitor endothelial vitro stimulator prostacyclin production, induces 3-kilobase Cox transcript cultured human umbilical vein cells (HUVEC). In contrast, cell mitogen, heparin-binding (acidic fibroblast) factor-1 (HBGF-1) inhibits synthesis HUVEC. this report, we describe effect HBGF-1 on mRNA Cells presence express diminished levels whereas quiescent maintained serum expressed 7-fold higher level for Cox. Concomitantly, translation product are also reduced HBGF-1. Further, HBGF-1, heparin, down-regulates dose- time-dependent manner. onset action slow, requiring up to 24 h depress mRNA. Lastly, effective dose similar required mitogenesis, suggesting proliferation may be reduction vitro. Thus, belong class genes reversibly down-regulated during periods proliferation.