Activation of the Mitochondrial Apoptotic Pathway in A Rat Model of Central Retinal Artery Occlusion.

摘要: PURPOSE. Apoptosis is known to play a role in cell death transient retinal ischemia. Little about the specific molecular pathways involved. The purpose of current study was evaluate rat model central artery occlusion (CRAO) that simulates clinical features CRAO humans and elucidate whether mitochondrial apoptotic pathway METHODS. induced by intravenous injection rose bengal green laser irradiation artery. documented at 1, 3, 6, 24 hours after irradiation. Changes Bax (proapoptotic Bcl-2-associated X protein), cytochrome c, caspase-9 cleavage cytosolic fractions neural tissues were measured Western blot analysis. within retina examined terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). RESULTS. Complete induced; however, became incomplete with spontaneous reperfusion branch arteries, starting 3 Only one or two arteries remained occluded 24-hour time point. Time-dependent, changes observed inner outer layers. analysis revealed translocation from cytoplasm, peaking 6 This accompanied accumulation c caspase-9. CONCLUSIONS. highly relevant manifestations an ideal animal for research. These findings indicate activation ischemic CRAO. provides better understanding ischemia-induced apoptosis. Antiapoptosis therapy directly targeting other diseases may be beneficial.