HPMA Copolymer-Anticancer Drug-OV-TL16 Antibody Conjugates. 1. Influence of the Method of Synthesis on the Binding Affinity to OVCAR-3 Ovarian Carcinoma Cells in Vitro
作者： V. Omelyanenko , P. Kopečková , C. Gentry , J.-G. Shiah , J. Kopeček
DOI: 10.3109/10611869608996827
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摘要: The influence of different methods binding the OV-TL16 antibody and its Fab' fragment to N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer--drug (adriamycin [ADR] or meso chlorin e6 mono(N-2-aminoethylamide) (Mce6)) conjugates on affinity an ovarian carcinoma (OVCAR-3) cell associated antigen was investigated. HPMA (ADR Mce6) via amino groups resulted in which were heterogeneous their binding. Coupling, copolymer--Mce6 conjugate carbohydrate region with a more homogeneous distribution constants than prepared by linking polymer groups. However, both decrease constant compared native antibody. Conjugates frgment demonstrated homogenous either whole To verify hypothesis that changes homogeneity are consequence conformational structure, series physiocochemical employed characterize conjugates. excitation energy transfer between drugs spectral properties Mce6 used monitor interactions drugs. quenching intrinsic fluorescence also study changes. An attempt has been made correlate biorecognition at cellular surface drug molecule conformation.
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