Mutations in the RAS and PI3K pathways are associated with metastatic location in colorectal cancers.

摘要: Background and objectives Identification of mutations in the downstream epidermal growth factor receptor (EGFR) signaling pathway could provide important insights EGFR-targeted therapies colorectal cancers. We analyzed mutation spectra PI3K/PTEN/AKT RAS/RAF/MAPK pathways cancers associations these with sites metastases or recurrence. Methods The study population comprised 1,492 retrospectively collected stages I–IV cancer specimens. Tissue was obtained between 2000 2010 at a single hospital. 61 hot spots using MALDI–TOF mass spectrometry for nucleic acid analysis. Results Mutations were found RAS 47.3% patients PI3K 14.3% patients, 9.2% carrying both pathways. Both significantly associated proximal tumors, mucinous microsatellite instability. Tumors exhibited higher frequency lung peritoneal metastasis than did tumors wild-type gene (P = 0.025 0.009, respectively). NRAS (P = 0.001). Conclusions Somatic primary tumor can influence patterns recurrence. J. Surg. Oncol. 2015 111:905–910. © 2014 Wiley Periodicals, Inc.