Direct determination of antibacterial norfloxacin in urine by isopotential fluorimetry

摘要: A novel and simple method is presented for the determination of norfloxacin in human urine by matrix isopotential synchronous fluorescence spectrometry (MISF). This useful compounds samples with unknown background fluorescence, such as urine, without need tedious sample pre-treatment. The was performed ethanol-water medium (10% v/v), at an apparent pH 4.8 provided adding sodium acetate-acetic acid buffer solution. In fluorescent intensity varied linearly its concentration from 20 to 200 ng/mL. An exhaustive statistical analysis has been developed all calibration graphs, this treatment includes robust regression least median squares, which also detects outliers leverage points. overall least-squares applied find more exact straight line that fits experimental data. error propagation considered calculate detection limit repeatability method. shows very low limits acceptable recoveries precisions. applicability proposed illustrated