Analysis of PTEN/MMAC1 Alterations in Aerodigestive Tract Tumors

摘要: PTEN/MMAC1 is a candidate tumor suppressor gene recently identified at chromosomal band 10q23. It mutated in sporadic brain, breast, and prostate cancer the germ line of patients with hereditary Cowden disease. We searched for genetic alterations 39 primary head neck cancers (HNSCCs), 42 non-small cell lung (NSCLCs), 80 pancreatic xenografts, 37 lines xenografts from colon, lung, gastric cancers. Microsatellite analysis revealed loss heterozygosity markers near 41% HNSCCs, 50% NSCLCs, 39% Three cases HNSCCs displayed homozygous deletion involving gene. sequenced entire coding region remaining tumors displaying found one terminating mutation HNSCC sample. Thus, second inactivation event was observed 4 cases. By use protein truncation assay, also eight NSCLC lines. Our results suggest that plays role genesis some types.