Human papillomavirus in transitional cell carcinoma of the urinary bladder

摘要: The presence of human papillomavirus (HPV) was detected in transitional cell carcinoma the urinary bladder. PCR amplification DNA from 71 tumors, using consensus primers for a fragment L1 gene, 6 strongly positive tumors (through ethidium bromide staining gel) and 22 moderately Southern blotting amplified DNA) total 28 (39%) tumors. HPV correlated with grade but not stage Typing performed on 31 tumors: all contained 16 except one Ta tumor which 11 DNA. Our data also showed large variability sensitivity detection, depending sample fixation, preparation, conditions, may explain part discrepancies reported literature association to bladder cancer. Because low copy number observed our results suggest that should ideally be tested fresh or frozen material, SDS detergent avoided preparation DNA, conditions are critical optimal detection.