The novel adipokine progranulin counteracts IL-1 and TLR4-driven inflammatory response in human and murine chondrocytes via TNFR1.

摘要: Progranulin (PGRN) is a recently identified adipokine that supposed to have anti-inflammatory actions. The proinflammatory cytokine interleukin-1β (IL1β) stimulates several mediators of cartilage degradation. Toll like receptor-4 (TLR4) can bind various damage-associated molecular patterns, leading inflammatory condition. So far, no data exist PGRN effects in conditions induced by IL1β or lipopolysaccharide (LPS). Here, we investigated the potential IL1β- LPS-induced responses chondrocytes. Human osteoarthritic chondrocytes and ATDC-5 cells were treated with presence not LPS. First, showed recombinant had on cell viability. We present evidence expression was increased during differentiation line. Moreover, mRNA protein cartilage, synovial infrapatellar fat pad tissue samples from OA patients. levels are upregulated under TNFα stimulation. Our able significantly counteract IL1β-induced NOS2, COX2, MMP13 VCAM-1. NOS2 also decreased PGRN. These mediated, at least part, through TNFR1. Taken together, our results suggest has clear function.