Immunoglobulin repertoire of B lymphocytes infiltrating breast medullary carcinoma.

摘要: Tumor specific peptides recognized by T lymphocytes infiltrating solid tumors, as well the corresponding cell receptor (TcR) repertoire usage, have been extensively investigated. By contrast, tumor B cells and their immunoglobulin (Ig) studied only in a limited number of tumors. The objective present study was to determine, whether DNA sequence analysis expressed variable regions that infiltrate breast cancer, could be used reveal potential binding capacity antibodies. To answer this question, about 200 Ig heavy (VH) light chain gene (VL) were cloned, sequenced comparatively analysed from typical medullary beast carcinoma (MBC), where massive plasma infiltration correlates with favourable prognosis despite its high grade. sequences classified into clusters, families subgroups, based on identity level germline, showing pattern oligoclonality. Some overrepresented clusters determined. In course detailed search Blastn database, VH VL showed more than 99% homology region, proved antigen capacity. Our data suggest, binder might selected using region analysis. This new approach benefit for further antibody engineering, difficulties binders phage library selection reduced time shortened.