Novel ganglioside antigen identified by B cells in human medullary breast carcinomas : The proof of principle concerning the tumor-infiltrating B lymphocytes
作者： Beatrix Kotlan , Peter Simsa , Jean-Luc Teillaud , Wolf Herman Fridman , Jozsef Toth
DOI: 10.4049/JIMMUNOL.175.4.2278
关键词:
摘要: The potential tumor-recognizing capacity of B cells infiltrating human breast carcinoma is an important aspect cancer biology. As experimental system, we used medullary because its heavy lymphocytic infiltration paralleled to a relatively better prognosis. Ig-rearranged V region VH-JH, Vκ-Jκ, and Vλ-Jλ genes, amplified by RT-PCR the cells, were cloned, sequenced, subjected comparative DNA analysis. A combinatorial single-chain variable fragment Ab minilibrary was constructed out randomly selected VH Vκ clones tested for binding activity. Our data analysis revealed that some sequences being assigned clusters with oligoclonal predominance, while other characteristics repertoire defined also. tumor-restricted binder clone could be κ against membrane fractions primary tumor cell lines, which proved overexpressed VH3-1 cluster. specific confirmed FACS MDA-MB 231 line. ELISA thin layer chromatography dot-blot experiments showed this target Ag ganglioside D3 (GD3). results are proof principle about carcinomas reveal key cancer-related Ags, such as GD3. GD3-specific Abs may influence progression further development diagnostic and/or therapeutic purposes.
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