Mechanisms of Mucosal and Parenteral Tuberculosis Vaccinations: Adenoviral-Based Mucosal Immunization Preferentially Elicits Sustained Accumulation of Immune Protective CD4 and CD8 T Cells within the Airway Lumen

摘要: The mechanisms underlying better immune protection by mucosal vaccination have remained poorly understood. In our current study we investigated the which respiratory virus-mediated provides remarkably against pulmonary tuberculosis than parenteral vaccination. A recombinant adenovirus-based (TB) vaccine expressing Mycobacterium Ag85A (AdAg85A) was administered either intranasally (i.n.) or i.m. to mice, and Ag-specific CD4 CD8 T cell responses, including frequency, IFN-γ production, CTL, were examined in spleen, lung interstitium, airway lumen. Although immunization with AdAg85A led activation of cells, particularly spleen and, a lesser extent, it failed elicit any response contrast, although i.n. effectively activate cells uniquely elicited higher numbers lumen that capable production cytolytic activities, as assessed an intratracheal vivo CTL assay. These luminal immunized mice splenic upon transfer locally lungs naive SCID conferred M. challenge. Our has demonstrated population plays important role TB, thus providing mechanistic insights into superior TB