Effects of egg yolk and cooling rate on the survival of refrigerated red deer (Cervus elaphus hispanicus) epididymal spermatozoa.
作者： M Rocío Fernández‐Santos , Milagros C Esteso , Ana J Soler , Vidal Montoro , José Julián Garde
DOI: 10.1111/J.1439-0531.2006.00649.X
关键词:
摘要: Egg yolk is a common component to sperm refrigeration for most of the deer species, role which protect membranes against cold shock. In addition, there have been many studies conservation ejaculated semen from stags, but few reported epididymal spermatozoa. This work was designed investigate combined effects cooling rates (slow: 0.23 degrees C/min vs rapid: 4.2 C/min) room temperature 5 C, and egg-yolk concentration (0, or 20%) in extender on survival Iberian red spermatozoa refrigerated at C. Heterospermic samples were diluted final approximately 400x10(6) sperm/ml with Tris-citrate-fructose (TCF)-egg-yolk diluent. Sperm quality vitro judged by microscopic assessments individual motility [sperm index (SMI)], plasma membrane (hypo-osmotic swelling test) acrosome (NAR) integrities. Our results first showed that presence egg significantly improves (p=0.01) viability after dilution. integrities post-refrigeration did not differ between procedures; however, SMI differed procedures 46.6% 50.0%; p=0.01). also (p<0.01) affected procedure, being rapid 20% suitable combination refrigeration. conclusion, improved characteristics Rapid protocol using TCF cooling.
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