It takes four to tango: Long noncoding RNA PANDA, SAF-A, polycomb repressive complexes and NF-Y in senescence regulation

摘要: Cellular senescence is a stable cell cycle arrest that inhibits the outgrowth of pre-cancerous cells but also implicated in wound healing, embryonic development, aging and age-related pathologies. Our knowledge on gene regulatory circuits establish maintain phenotype highly fragmentary. Here, we provide several lines evidence supporting critical novel function scaffolding-attachment-factor A SAF-A long, noncoding RNA PANDA establishment maintenance phenotype. First, demonstrate are differentially expressed presenescent compared to senescent cells. Second, show both actively contribute induction maintenance. Finally, physically functionally interact directly repress senescence- proliferation-promoting genes by regulating access polycomb repressive complexes PRC1 PRC2 as well transcription factor NF-YA their cognate target genes. Together, our data identify DNA-RNA-binding protein key actors fate decision unravel importance dependent changes factors RNAs.